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A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a. Typically the object is (secured) on the slide, and then both are inserted together in the microscope for viewing. This arrangement allows several slide-mounted objects to be quickly inserted and removed from the microscope, labeled, transported, and stored in appropriate slide cases or folders etc. Microscope slides are often used together with a cover slip or cover glass, a smaller and thinner sheet of glass that is placed over the specimen.

Slides are held in place on the microscope's stage by slide clips, slide clamps or a cross-table which is used to achieve precise, remote movement of the slide upon the microscope's stage (such as in an automated / computer operated system, or where touching the slide with fingers is inappropriate either due to the risk of contamination or lack of precision). Common dimensions of microscope slides (in mm). A standard microscope slide measures about 75 mm by 25 mm (3″ by 1″) and is about 1 mm thick. A range of other sizes are available for various special purposes, such as 75 x 50 mm and for use, 46 x 27 mm for studies, and 48 x 28 mm for. Slides are usually made of common glass and their edges are often or polished. Microscope slides are usually made of optical quality, such as or, but specialty plastics are also used. Slides are often used when transparency is important, e.g.

While plain slides are the most common, there are several specialized types. A concavity slide or cavity slide has one or more shallow depressions ('wells'), designed to hold slightly thicker objects, and certain samples such as liquids. Slides may have rounded corners for increased safety or robustness, or a cut-off corner for use with a slide clamp or cross-table, where the slide is secured by a spring-loaded curved arm contacting one corner, forcing the opposing corner of the slide against a right angled arm which does not move. If this system were used with a slide which did not incorporate these cut-off corners, the corners would chip and the slide could shatter.

A graticule slide is marked with a of lines (for example, a 1 mm grid) that allows the size of objects seen under magnification to be easily estimated and provides reference areas for counting minute objects. Sometimes one square of the grid will itself be subdivided into a finer grid. Slides for specialized applications, such as for cell counting, may have various reservoirs, channels and barriers or on their upper surface.

Various permanent markings or masks may be,, or deposited on the surface by the manufacturer, usually with inert materials such as. Microscope slides with prepared, stained, and labeled tissue specimens in a standard 20-slide folder. The mounting of specimens on microscope slides is often critical for successful viewing. The problem has been given much attention in the last two centuries and is a well-developed area with many specialized and sometimes quite sophisticated techniques. Specimens are often held into place using the smaller glass cover slips.

The main function of the cover slip is to keep solid specimens pressed flat, and liquid samples shaped into a flat layer of even thickness. This is necessary because microscopes have a very narrow within which they focus. The cover glass often has several other functions. It holds the specimen in place (either by the weight of the cover slip or, in the case of a wet mount, by ) and protects the specimen from dust and accidental contact. It protects the microscope's from contacting the specimen and vice versa; in or the cover slip prevents contact between the immersion liquid and the specimen. The cover slip can be glued to the slide so as to seal off the specimen, retarding and of the specimen and also preventing contamination. A number of sealants are in use, including commercial sealants, laboratory preparations, or even regular clear, depending on the sample.

A solvent-free sealant that can be used for live cell samples is 'valap', a mixture of, and in equal parts. And can be grown directly on the cover slip before it is placed on the slide, and specimens may be permanently mounted on the slip instead of on the slide. Cover slips are available in a range of sizes and thicknesses.

Using the wrong thickness can result in and a reduction in resolution and image intensity. Specialty objectives may used to image specimens without coverslips, or may have correction collars that permit a user to accommodate for alternative coverslip thickness. Dry mount [ ] In a dry mount, the simplest kind of mounting, the object is merely placed on the slide. A cover slip may be placed on top to protect the specimen and the microscope's objective and to keep the specimen still and pressed flat. This mounting can be successfully used for viewing specimens like pollen, feathers, hairs, etc. It is also used to examine particles caught in transparent (e.g., in analysis of airborne ).